Autoimmune diseases are characterised by specific antibody patterns. The IMTECH Line Immune Assays (LIA) have been developed for rapid antibody profile testing. Up to 17 different autoantibodies can be tested in a single run. Therefore the antigens of the LIAs are spotted in lines on a nitrocellulose membrane. Bound autoantibodies are visualised by using specific HRP conjugated antibody and substrate. Samples positive for autoantibodies are detected by presence of distinct brown lines.
Designed to optimise differential diagnosis of rheumatoid diseases: Systemic Lupus Erythematosus (SLE), Sjögren's syndrome (SS), CREST (calcinosis, Raynaud's, esophagealdysmotility, sclerodactyly and telangiectasia) syndrome, Systemic Scleroderma (Ssc) Mixed Connective Tissue Diseases (MCTD), Primary Biliary Cirrhosis (PBC) and Myositis.
ANA-LIA enables the detection of 12 different anti-nuclear antibodies (ANA).ANAs are found in systemic inflammatory rheumatic diseases like mixed connective tissue diseases (MCTD), systemic lupus erythematosus (SLE), Sjögren's syndrome and are also indicative of autoimmune hepatitis(AIH).
The Myositis-LIA enables the screening of 7 autoantibodies that are specific for myositis and myositis-related syndromes.
Liver-LIA is designed for fast and reliable differential diagnosis of autoimmune hepatitis.Vasculitis-LIA is designed for the differential diagnosis of autoimmune vasculitis. Anti-neutrophil cytoplasmic autoantibodies (ANCA) associated diseases like Wegener’s granulomatosis, Churg-Strauss syndrome (eosinophilicgranulomatosis with polyangiitis, EGPA) or microscopic polyangiitis can be detectedby testing for autoantibodies targeting proteinase 3 (PR3, cANCA) or myeloperoxidase (MPO, pANCA). Glomerularbasement membrane (GBM) autoantibodies are specific for the diagnosis of Goodpasture’s syndrome.
Gastro-LIA is used for detection of IgA and IgG autoantibodies of autoimmune gastrointestinal diseases.Both d-gliadin (DGP) and tissue transglutaminase (tTG) are specific autoantibody markers for Celiac disease. Anti-saccharomyces cerevisiaemannan antibodies (ASCA) are seen in Crohn’s disease. Perniciousanemia can be detected by anti-parietal cell antibodies (PCA) and intrinsic factor (IF) autoantibodies.